| 胡慧敏,李 倩,王振宇,尚 艳,朱 艳,赵元寿.壳聚糖激活类黄酮生物合成途径增强马铃薯抗干腐病能力[J].保鲜与加工,2025,25(10):46~56 |
| 壳聚糖激活类黄酮生物合成途径增强马铃薯抗干腐病能力 |
| Chitosan Treatment Enhanced the Resistance of Potato Tuber to Dry Rot by Activating Flavonoid Biosynthesis Pathway |
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| DOI： |
| 中文关键词: 壳聚糖 马铃薯 干腐病 类黄酮生物合成途径 |
| 英文关键词:chitosan potato dry rot flavonoid biosynthetic pathway |
| 基金项目:甘肃省青年科技基金项目（23JRRA1473） |
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| 中文摘要: |
| 为明确壳聚糖处理在马铃薯块茎抗干腐病过程中对类黄酮合成途径的调控机制，以马铃薯块茎为试 材，采用壳聚糖处理联合接骨木镰刀菌（Fusarium sambucinum）挑战接种，研究其对马铃薯块茎的品质指标、总 酚含量、类黄酮含量以及类黄酮生物合成途径关键酶活性的影响。结果表明：壳聚糖处理显著降低了马铃薯 块茎的失重率、病斑面积和病斑深度，提高了块茎硬度，降低了色度变化值，推迟了呼吸高峰的出现并降低了其 峰值。此外，该处理还促进了马铃薯块茎类黄酮的积累，提高了苯丙氨酸解氨酶（Phenylalnine ammonialyase， PAL）、肉桂酸-4-羟化酶（Cinnamic acid-4-hydroxylase， C4H）、4-香豆酰-辅酶A连接酶（4-Coumarate coenzyme A ligase， 4CL）、查尔酮合成酶（Chalcone synthase， CHS）和查尔酮异构酶（Chalcone isomerase， CHI）的活性；壳聚糖处理后再进行F. sambucinum挑战接种可进一步促进块茎总酚和类黄酮含量的增加，显著 上调了PAL、C4H、4CL、CHS和CHI活性。壳聚糖处理通过激活类黄酮代谢途径，提高相关酶活性，促进总酚 和类黄酮积累，通过诱导体内的抗病因子，增强了马铃薯块茎对干腐病的抗性。 |
| 英文摘要: |
| To clarify the regulatory mechanism of chitosan treatment on the flavonoid biosynthesis pathway during the process of potato tubers resisting dry rot disease, the effects of chitosan treatment combined with challenge inoculation of Fusarium sambucinum on the quality indicators, total phenolic content, flavonoid content, and the activities of key enzymes in the flavonoid biosynthesis pathway of potato tubers were studied. The results showed that after chitosan treatment, the weight loss rate, spot area and spot depth of tuber caused by F. sambucinum challenge inoculation were significantly reduced, the tuber hardness was increased, the color change value was decreased, and the appearance of respiratory peak was delayed and reduced. Chitosan treatment facilitated the accumulation of flavonoids and enhanced the activities of PAL, C4H, 4CL, CHS, and CHI in potato tubers. At the same time, chitosan treatment could further increase the contents of total phenols and flavonoids in tubers after F. sambucinum challenge inoculation, and signifi?cantly upregulated the activities of PAL, C4H, 4CL, CHS and CHI. Chitosan treatment enhanced the resistance of potato tubers to dry rot by activating flavonoid metabolic pathway, improving the activity of related enzymes, promoting the accumulation of total phenols and flavonoids, and inducing disease-resistant factors in vivo. |
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