| 张翼斐,鲁丁强,刘玉姣,毕祎萌,关文强.温度对采后番茄代谢通量的影响研究[J].保鲜与加工,2025,25(10):36~45 |
| 温度对采后番茄代谢通量的影响研究 |
| Effects of Temperature on Metabolic Fluxes in Postharvest Tomatoes |
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| DOI： |
| 中文关键词: 番茄 温度 果糖 代谢通量 中心代谢 |
| 英文关键词:tomato temperature fructose metabolic flux central metabolism |
| 基金项目:天津市科技计划项目（23JCZDJC00690） |
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| 摘要点击次数: 141 |
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| 中文摘要: |
| 为了解贮藏温度对番茄采后代谢途径中果糖和乳酸代谢通量的影响，以进一步优化番茄贮 藏条件，在不同温度梯度（-2、0、4、8、12、16、20、24、28、30 ℃）下贮藏番茄24 h，测定其葡萄糖、果糖、 乳酸和还原型烟酰胺腺嘌呤二核苷酸（NADH）的变化速率并计算相关代谢通量，同时还测定了番茄 在8、12、16、25 ℃下贮藏期间的失重率、腐烂率、可溶性固形物（TSS）含量、颜色、硬度、VC含量、过氧 化物酶（POD）活性和多酚氧化酶（PPO）活性的变化。结果表明：相较其他温度，在12 ℃下番茄具有 较高的果糖和乳酸代谢通量，能够保持较高的合成代谢和较低水平的分解代谢；该温度不仅有效降 低了果实失重率、软化速率和腐烂率，减缓了可溶性固形物和 VC 等物质的流失，还抑制了 POD 和 PPO 活性，从而维持低水平的呼吸代谢。该研究对了解番茄采后贮藏代谢机制及选择合适贮藏温 度具有重要意义。 |
| 英文摘要: |
| In order to investigate the effect of storage temperature on the metabolic fluxes of fructose and lactate in postharvest metabolic pathways of tomatoes and optimize storage conditions, the rates of change of glucose, fructose, lactate and reduced nicotinamide adenine dinucleotide (NADH) were measured and the related metabolic fluxes were calculated after 24 h of storage at different temperature gradients (-2, 0, 4, 8, 12, 16, 20, 24, 28, 30 ℃). Additionally, the rate of weight loss, decay rate, total soluble solid (TSS) content, color, hardness, VC content, peroxidase (POD) activity, and phenol oxidase (PPO) activity were also determined during storage at 8, 12, 16, and 25 ℃. The results showed that compared to other temperatures, tomatoes had higher metabolic fluxes of fructose and lactic acid at 12 ℃, and could maintain higher anabolic metabolism and lower levels of catabolic metabolism; this temperature not only effectively reduced weight loss rate, softening rate and decay rate of the fruits, and slowed down the deterioration of the loss of TSS and VC, and suppressed the activities of POD and PPO, to maintain a low level of respiratory metabolism. This study is of great significance for understanding the metabolic mechanism of postharvest storage of tomatoes and selecting the appropriate storage temperature. |
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